in vitro inhibition of human sperm creatine kinase by nicotine, cotinine and cadmium, as a mechanism in smoker men infertility
نویسندگان
چکیده
background: nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. in the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase activity in vitro. materials and methods: total creatine kinase activity is measured in sperm homogenates after chromatography on a diethylaminoethyl cellulose (deae-32) column. results: we show that creatine kinase activity is significantly inhibited by nicotine (44%), cotinine (39%) and cadmium (65%) at a concentration of 60 μg/ml. kinetic studies reveal that the inhibitory effect of nicotine, cotinine and cadmium are competitive in relation to creatine phosphate. conclusion: considering the importance of creatine kinase activity for normal sperm energy metabolism, our results suggest that inhibition of this enzyme by nicotine, cotinine and cadmium may be an important mechanism in infertility amongst male smokers. however, further investigations are needed to elucidate the exact mechanism of cigarette effect on male reproductive function at the molecular level.
منابع مشابه
In Vitro Inhibition of Human Sperm Creatine Kinase by Nicotine, Cotinine and Cadmium, as a Mechanism in Smoker Men Infertility
Background Nicotine, cotinine and cadmium are harmful components of cigarettes that have an effect on human reproductive function. Although the effects of cigarette smoke on male reproductive function is characterized in several articles its mechanism of action is still unknown. In the present study, we investigate the effect of nicotine, cotinine and cadmium on human sperm creatine kinase acti...
متن کاملIn vitro Effect of Lead, Silver, Tin, Mercury, Indium and Bismuth on Human Sperm Creatine Kinase Activity: a Presumable Mechanism for Men Infertility
Background: The aim of the present study was to investigate the in vitro effects of mercury (Hg+2), lead (Pb+2), silver (Ag+2), tin (Sn+2), bismuth (Bi+3) and indium (In+3) ions on sperm creatine kinase. Methods: creatine kinase was isolated from human sperm homogenates after chromatography on a DEAE cellulose column. Results: At 60 µg ml-1 metal concentration, 70% of the creatine kinase activi...
متن کاملin vitro effect of lead, silver, tin, mercury, indium and bismuth on human sperm creatine kinase activity: a presumable mechanism for men infertility
background: the aim of the present study was to investigate the in vitro effects of mercury (hg+2), lead (pb+2), silver (ag+2), tin (sn+2), bismuth (bi+3) and indium (in+3) ions on sperm creatine kinase. methods: creatine kinase was isolated from human sperm homogenates after chromatography on a deae cellulose column. results: at 60 µg ml-1 metal concentration, 70% of the creatine kinase activi...
متن کاملThe Effect of Cigarette Smoking on Human Sperm Creatine Kinase Activity: As An ATP Buffering System in Sperm
متن کامل
In vitro effect of lead, silver, tin, mercury, indium and bismuth on human sperm creatine kinase activity: a presumable mechanism for men infertility.
BACKGROUND The aim of the present study was to investigate the in vitro effects of mercury (Hg+2), lead (Pb+2), silver (Ag+2), tin (Sn+2), bismuth (Bi+3) and indium (In+3) ions on sperm creatine kinase. METHODS creatine kinase was isolated from human sperm homogenates after chromatography on a DEAE cellulose column. RESULTS At 60 mug ml-1 metal concentration, 70% of the creatine kinase acti...
متن کاملthe in-vitro effects of nicotine, cotinine and leptin on sperm parameters analyzed by casa system
backgrand: cotinine (cot) is a major degradation product of nicotine (nic). the participation of leptin in female reproduction is well-established, but any role in male reproductive function is at the best tenuous. objective: the aim of this study was to evaluate the in-vitro effects of nicotine, cotinine and leptin on sperm parameters in normal semen of non-smokers fertile men. materials and m...
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عنوان ژورنال:
international journal of fertility and sterilityجلد ۲، شماره ۳، صفحات ۱۲۵-۱۳۰
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